New arrivals and sick horses should be quarantined/isolated for a minimum of 14 days, ideally 30 days.
Isolating new arrivals and sick horses allows you to properly monitor said horse while keeping the others in your herd safe. While horse is isolated you should monitor his manure, feed/water intake, for any signs of sickness/disease and make sure to do a daily health check. By keeping new arrivals and sick horses in quarantine you are preventing the spread of sickness and disease to your main herd as well as making sure these new arrivals don’t catch anything for the residing herd as well.
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